ser73 phosphorylated c jun  (Cell Signaling Technology Inc)

Journal: Cell Reports Medicine

Article Title: Arrestin-3-assisted activation of JNK3 mediates dopaminergic behavioral sensitization

doi: 10.1016/j.xcrm.2024.101623

Figure Lengend Snippet: Changes in the JNK-dependent c-Jun activation associated with the loss of Arr3 (A) Representative western blots showing the levels of c-Jun phosphorylated at the JNK site Ser73 (upper panel) and total c-Jun (middle panel). (B) Quantification of the western blot data for phospho-c-Jun. Significant hemisphere × group interaction ( p < 0.001) is due to elevated c-Jun phosphorylation in the lesioned striatum of L-DOPA-treated WT mice not evident in other groups. n = 6–9. (C) High power images of the mouse brain double-stained for phospho-c-Jun (green) and TH (red). (D) Representative western blots showing phosphorylated and total c-Jun. (E) Quantification of the western blot data for phospho-c-Jun. One-way ANOVA yielded significant effect of group ( p = 0.007). ∗∗ - p < 0.01 by post hoc Tukey’s test. (F) Low power images of sections co-stained for TH (red) and phospho-c-Jun (green).

Article Snippet: Active JNK was detected with rabbit antibodies to doubly phosphorylated (Thr183/Tyr185) JNK (Cell Signaling, catalog # 4668 or 9251; 1: 1,000). c-Jun phosphorylated at the JNK phosphorylation site Ser73 was detected with a phospho-specific rabbit antibody (Cell Signaling, # 3270; 1:1,000) and the total c-Jun – with mouse monoclonal antibody (Cell Signaling, # 2315; 1:1,000).

Techniques: Activation Assay, Western Blot, Phospho-proteomics, Staining